Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJEB11827

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This environmental metagenome amplicon sequencing via 454 GS FLX Titanium provides untargeted microbial/eukaryotic community surveying (1.3 million reads, 531 MB bases) with modest per-base accuracy (Q30=80.2%)—454 chemistry permits longer reads suitable for homopolymer-poor marker genes (e.g., 16S rRNA) but suffers from systematic length-dependent errors requiring specialized quality filtering and consensus calling.

Data type / assay
amplicon
Organism
metagenome
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
57 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 531871692 reported
total reads 1337184 reported
n content pct 0.702 measured
pct q20 bases 92 measured
pct q30 bases 80.2 measured
gc content pct 47.4 measured
mean read length 385.4 measured
mean base quality 35.1 measured
adapter content pct 0 measured
duplication rate pct 96.69 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.2 measured ×1 51%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0