Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJEB12221

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

63/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human metagenome amplicon sequencing via 454 GS FLX Titanium with 789,400 reads and 295 million bases at modest quality (93.4% Q20, 78.9% Q30). The longer read length inherent to 454 pyrosequencing allows better phylogenetic resolution but lower per-base accuracy. Users should note the read type is unknown (low confidence) and verify whether this older-platform data is compatible with modern bioinformatic pipelines designed for Illumina amplicons.

Data type / assay
amplicon
Organism
human metagenome
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
9 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 295049592 reported
total reads 789400 reported
n content pct 0.014 measured
pct q20 bases 93.4 measured
pct q30 bases 78.9 measured
gc content pct 55.1 measured
mean read length 279.1 measured
mean base quality 34.4 measured
adapter content pct 0 measured
duplication rate pct 77.03 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 63/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.9 measured ×1 45%
adapter content pct 0 measured ×0.5 100%
QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0