Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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PRJEB12522

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Homo sapiens amplicon sequencing via 454 GS Junior provides targeted coverage (78K reads, 31.5 MB bases) with modest per-base accuracy (Q30=71.5%)—the small throughput and lower quality typical of 454 Junior hardware limit sensitivity for rare-variant or low-abundance allele detection, best suited for single-locus or few-locus confirmation studies.

Data type / assay
amplicon
Organism
Homo sapiens
Instrument
454 GS Junior
Platform
LS454
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
18 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 31504505 reported
total reads 77988 reported
n content pct 0.003 measured
pct q20 bases 92.2 measured
pct q30 bases 71.5 measured
gc content pct 53.2 measured
mean read length 369.3 measured
mean base quality 33.7 measured
adapter content pct 0 measured
duplication rate pct 95.92 measured
mean target coverage 0.5 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 71.5 measured ×1 8%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0