PRJEB12583

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Nicrophorus vespilloides RNA-seq with good base quality (Q30 91.3%, base quality 35.6) constrained by elevated duplication (45.6%) suggesting transcript complexity collapse or cDNA synthesis bias; usable for expression profiling with duplication-aware quantification but risky for isoform-level work.

Data type / assay

bulk-RNA-seq

Organism

Nicrophorus vespilloides

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

6 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 9600000000 reported

total reads 96000000 reported

n content pct 0.003 measured

pct q20 bases 97.3 measured

pct q30 bases 91.3 measured

gc content pct 45.8 measured

mean read length 100 measured

mean base quality 35.6 measured

adapter content pct 1.21 measured

duplication rate pct 45.62 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.3 measured ×1 100%

mean base quality 35.6 measured ×0.6 100%

adapter content pct 1.21 measured ×0.4 100%

duplication rate pct 45.62 measured ×0.4 65%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0