PRJEB12612

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus bulk RNA-seq via Illumina HiSeq 2500 with 170.3 million reads and 21.4 billion bases at solid quality (96.6% Q20, 93.4% Q30). The 50.1% GC and low N-content (0.001%) indicate clean library construction. This mouse dataset is suitable for studying tissue-specific and developmental transcriptomics with good sensitivity to moderately expressed genes.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

6 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 21463221402 reported

total reads 170343027 reported

n content pct 0.001 measured

pct q20 bases 96.6 measured

pct q30 bases 93.4 measured

gc content pct 50.1 measured

mean read length 126 measured

mean base quality 36.2 measured

adapter content pct 8.75 measured

duplication rate pct 32.82 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.4 measured ×1 100%

mean base quality 36.2 measured ×0.6 100%

adapter content pct 8.75 measured ×0.4 63%

duplication rate pct 32.82 measured ×0.4 94%

QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0