PRJEB15336

BioProject

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Large-scale mouse transcriptome via Illumina HiSeq 2500 with 101 bp reads, excellent quality (98.6% Q20), and very high depth (950M reads), enabling comprehensive isoform and expression profiling. Robust coverage supports sensitive detection across tissues and conditions relevant to developmental or disease models.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

34 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 96022237927 reported

total reads 950715227 reported

n content pct 0 measured

pct q20 bases 98.6 measured

pct q30 bases 96.5 measured

gc content pct 43.1 measured

mean read length 101 measured

mean base quality 40.2 measured

adapter content pct 0 measured

duplication rate pct 12.23 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.5 measured ×1 100%

mean base quality 40.2 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 12.23 measured ×0.4 100%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0