PRJEB19895

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Homo sapiens amplicon dataset from 454 GS FLX Titanium generated 222.9 thousand reads (74.7 megabases) with high quality (96.9% Q20, 88.2% Q30) and 54.1% GC. The 454 platform's longer reads provide improved amplicon-based phylogenetic resolution despite the smaller total read count compared to Illumina platforms.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

13 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 74689185 reported

total reads 222892 reported

n content pct 0.004 measured

pct q20 bases 96.9 measured

pct q30 bases 88.2 measured

gc content pct 54.1 measured

mean read length 283.5 measured

mean base quality 36.8 measured

adapter content pct 0 measured

duplication rate pct 93.23 measured

mean target coverage 1.2 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.2 measured ×1 91%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0