PRJEB24010

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

91/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Mus musculus bulk-RNA-seq from Illumina HiSeq 2500 generated 336.2 million short reads (48.8 gigabases) with good quality (92.3% Q20, 85.7% Q30) and 48.3% GC. The large-scale dataset enables comprehensive mouse transcriptome profiling and sensitive detection of lowly-expressed genes across tissues or conditions.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), BAM/CRAM (aligned)

N numbers (samples, groups)

95 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 48826551465 reported

total reads 336201747 reported

n content pct 0.007 measured

pct q20 bases 92.3 measured

pct q30 bases 85.7 measured

gc content pct 48.3 measured

mean read length 75 measured

mean base quality 34 measured

adapter content pct 0 measured

duplication rate pct 16.04 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 91/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.7 measured ×1 79%

mean base quality 34 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 16.04 measured ×0.4 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0