Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJEB24314

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

59/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Uncultured eukaryote amplicon sequencing compromised by below-threshold base quality (Q30 77.6%, Q20 88.9%) combined with very high duplication (96.9%); unsuitable for rare-variant discrimination or subtle taxa identification, though may suffice for coarse presence/absence assessment.

Data type / assay
amplicon
Organism
uncultured eukaryote
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
247 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 12192577649 reported
total reads 20366760 reported
n content pct 0.026 measured
pct q20 bases 88.9 measured
pct q30 bases 77.6 measured
gc content pct 44.5 measured
mean read length 299.1 measured
mean base quality 32.7 measured
adapter content pct 0 measured
duplication rate pct 96.88 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 59/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 77.6 measured ×1 38%
adapter content pct 0 measured ×0.5 100%
QC cost 3 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0