PRJEB24565

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Biofilm metagenome amplicon sequencing from Nanopore long-read technology completely failed quality thresholds with zero Q30 bases and base quality 8.9, rendering the dataset unsuitable for any taxonomic inference; represents a failed sequencing run requiring resequencing.

Data type / assay

amplicon

Organism

biofilm metagenome

Instrument

MinION

Platform

OXFORD_NANOPORE

Read type

long-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

19 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 604083425 reported

total reads 344966 reported

n content pct 0 measured

pct q20 bases 0.2 measured

pct q30 bases 0 measured

gc content pct 60.9 measured

mean read length 6222 measured

mean base quality 8.9 measured

adapter content pct 0.06 measured

duplication rate pct 1.88 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 0 measured ×1 0%

adapter content pct 0.06 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0