Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
← Dataset search

PRJEB30292

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

52/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human gut metagenome amplicon sequencing via Illumina MiSeq with 12.7 million reads and 6.36 billion bases but notably lower quality (83.3% Q20, 75.7% Q30) and 51.8% GC. The reduced base accuracy may increase false-positive OTU assignment or inflate richness estimates due to sequencing errors. Consider aggressive quality filtering and chimera-removal strategies before trusting low-abundance community members.

Data type / assay
amplicon
Organism
human gut metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
136 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 6361470002 reported
total reads 12672251 reported
n content pct 0 measured
pct q20 bases 83.3 measured
pct q30 bases 75.7 measured
gc content pct 51.8 measured
mean read length 251 measured
mean base quality 31.8 measured
adapter content pct 0 measured
duplication rate pct 87.05 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 52/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.7 measured ×1 28%
adapter content pct 0 measured ×0.5 100%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0