PRJEB33070

BioProject

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Deep RNA-seq of the ciliate *Paramecium tetraurelia* via Illumina HiSeq 4000 with high quality (98.7% Q20) and exceptional depth (802M reads), enabling sensitive transcript detection across developmental stages or starvation responses. Comprehensive coverage supports genome annotation refinement and novel transcript discovery in this protist model.

Data type / assay

bulk-RNA-seq

Organism

Paramecium tetraurelia

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

27 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 218814454206 reported

total reads 801990715 reported

n content pct 0.002 measured

pct q20 bases 98.7 measured

pct q30 bases 96.3 measured

gc content pct 39 measured

mean read length 138 measured

mean base quality 39.5 measured

adapter content pct 0 measured

duplication rate pct 49.07 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.3 measured ×1 100%

mean base quality 39.5 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 49.07 measured ×0.4 58%

QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0