Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJEB33281

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Whole-genome amplification sequencing of an uncultured alphaproteobacterium via Illumina NextSeq 500 with 150 bp reads and high quality (96.3% Q20), enabling genome-level phylogenetic and functional profiling of environmental bacteria. Limited depth reflects WGA throughput constraints; single-cell or low-biomass applications may benefit from resequencing.

Data type / assay
WGS
Organism
uncultured Alphaproteobacteria bacterium
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 878611181 reported
total reads 5879048 reported
n content pct 0.001 measured
pct q20 bases 96.3 measured
pct q30 bases 93.9 measured
gc content pct 31.9 measured
mean read length 149.4 measured
mean base quality 34.5 measured
adapter content pct 0 measured
duplication rate pct 51.38 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.9 measured ×1 100%
duplication rate pct 51.38 measured ×0.5 0%
adapter content pct 0 measured ×0.4 100%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0