PRJEB33511

BioProject

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

RNA-seq of bovine (*Bos taurus*) tissue via Illumina MiSeq with hybrid short+long reads and very low quality (43.7% Q20, 12.2% Q30), suggesting severely degraded library or technical failure. Data quality is marginal for reliable expression profiling; extensive filtering and qPCR validation essential before interpretation.

Data type / assay

bulk-RNA-seq

Organism

Bos taurus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

hybrid (short+long)

Files available

FASTQ (raw reads), BAM/CRAM (aligned)

N numbers (samples, groups)

61 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 65942416753 reported

total reads 52458846 reported

n content pct 0 measured

pct q20 bases 43.7 measured

pct q30 bases 12.2 measured

gc content pct 69 measured

mean read length 1003.9 measured

mean base quality 17.5 measured

adapter content pct 0 measured

duplication rate pct 6.21 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 12.2 measured ×1 0%

mean base quality 17.5 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 6.21 measured ×0.4 100%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0