Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJEB35531

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

69/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina MiSeq metatranscriptomics of gut microbiota delivers 38.5 billion bases at 92.6% Q20 and 83.3% Q30, providing high-confidence short-read RNA-seq from a complex community. This data answers questions about active gene expression across diverse bacterial taxa and can support functional profiling of the microbiome in health or disease contexts.

Data type / assay
bulk-RNA-seq
Organism
gut metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
727 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 38451412734 reported
total reads 66462067 reported
n content pct 0.009 measured
pct q20 bases 92.6 measured
pct q30 bases 83.3 measured
gc content pct 54.5 measured
mean read length 301 measured
mean base quality 34.1 measured
adapter content pct 1.17 measured
duplication rate pct 93.39 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 69/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.3 measured ×1 67%
mean base quality 34.1 measured ×0.6 100%
adapter content pct 1.17 measured ×0.4 100%
duplication rate pct 93.39 measured ×0.4 0%
QC cost 3 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0