PRJEB4256

BioProject first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Homo sapiens amplicon sequencing via 454 GS FLX with 185,239 reads and 101.6 million bases at lower quality (88.7% Q20, 66.6% Q30), read type unknown (low confidence). The 51.7% GC and elevated N-content (0.029%) reflect 454 limitations. This small, older-platform dataset is suitable only for exploratory human microbiota or targeted genetic studies and should be treated as preliminary without validation resequencing.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

16 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 101672614 reported

total reads 185239 reported

n content pct 0.029 measured

pct q20 bases 88.7 measured

pct q30 bases 66.6 measured

gc content pct 51.7 measured

mean read length 353.5 measured

mean base quality 31.7 measured

adapter content pct 0 measured

duplication rate pct 93.97 measured

mean target coverage 1.6 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 66.6 measured ×1 0%

adapter content pct 0 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0