Corpus 1,283 assessed · 1,184 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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PRJEB43658

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

92/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NextSeq 550 large-scale RNA-seq of Mus musculus produces 149 billion bases at 96% Q20 and 94.4% Q30 across 1.9 billion reads. Comprehensive mouse transcriptomics enabling genome-wide expression profiling and splicing-variant discovery.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
120 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 149167057951 reported
total reads 1976367594 reported
n content pct 0.005 measured
pct q20 bases 96 measured
pct q30 bases 94.4 measured
gc content pct 47.2 measured
mean read length 75.5 measured
mean base quality 34.4 measured
adapter content pct 0 measured
duplication rate pct 51.29 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 92/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.4 measured ×1 100%
mean base quality 34.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 51.29 measured ×0.4 53%
QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0