Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

PRJEB4677

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Gallus gallus. Grade B, 89/100 with all metrics measured. Severely elevated duplication (60.8%) is the critical limitation—representing heavy PCR bias that distorts transcript abundance and reduces effective sequencing depth. Q30 bases (93.3%) and quality (36) are excellent, but duplication restricts use to qualitative studies with strict deduplication and variance correction.

Data type / assay
bulk-RNA-seq
Organism
Gallus gallus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
27 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 377686040400 reported
total reads 1888430202 reported
n content pct 0.003 measured
pct q20 bases 97.7 measured
pct q30 bases 93.3 measured
gc content pct 55 measured
mean read length 100 measured
mean base quality 36 measured
adapter content pct 0.33 measured
duplication rate pct 60.81 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.3 measured ×1 100%
mean base quality 36 measured ×0.6 100%
adapter content pct 0.33 measured ×0.4 100%
duplication rate pct 60.81 measured ×0.4 32%
QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 80