PRJEB4713

BioProject

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

50/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

RNA-seq of the euglenid protist *Euglena gracilis* via 454 GS FLX Titanium with longer reads (~385 bp) but only 701k reads and modest quality (87.5% Q20). Low depth and older platform limit transcript abundance precision; primarily valuable as historical reference or for rare-transcript discovery.

Data type / assay

bulk-RNA-seq

Organism

Euglena gracilis

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

10 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 269770537 reported

total reads 701798 reported

n content pct 0.028 measured

pct q20 bases 87.5 measured

pct q30 bases 67.6 measured

gc content pct 53.5 measured

mean read length 412.6 measured

mean base quality 32.1 measured

adapter content pct 0 measured

duplication rate pct 4.23 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 50/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 67.6 measured ×1 0%

mean base quality 32.1 measured ×0.6 68%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 4.23 measured ×0.4 100%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0