Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

PRJEB5761

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina HiSeq 2000 metatranscriptomics of gut microbiota produces 365 million bases at 91.9% Q20 and 80.7% Q30, with notably low read count (~1.2 million). Sparse read depth suggests pilot or targeted sampling rather than comprehensive profiling, limiting detection of rare expressed genes.

Data type / assay
bulk-RNA-seq
Organism
gut metagenome
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
81 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 365817000 reported
total reads 1219390 reported
n content pct 0 measured
pct q20 bases 91.9 measured
pct q30 bases 80.7 measured
gc content pct 53.4 measured
mean read length 150 measured
mean base quality 32.1 measured
adapter content pct 0 measured
duplication rate pct 71.42 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.7 measured ×1 54%
mean base quality 32.1 measured ×0.6 68%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 71.42 measured ×0.4 8%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0