PRJEB5860

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Barley amplicon. Moderate reusability (grade C) with Q30 at 81.4% (scored 57/100), which is borderline acceptable for amplicon. Very high duplication (86.37%) is expected for targeted sequencing; reuse is conditional on whether your downstream analysis can tolerate the Q30 floor.

Data type / assay

amplicon

Organism

Hordeum vulgare

Instrument

454 GS FLX Titanium

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

8 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 45181595362 reported

total reads 227484964 reported

n content pct 0.009 measured

pct q20 bases 94.2 measured

pct q30 bases 81.4 measured

gc content pct 55.2 measured

mean read length 356.2 measured

mean base quality 35.3 measured

adapter content pct 0 measured

duplication rate pct 86.37 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.4 measured ×1 57%

adapter content pct 0 measured ×0.5 100%

QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0