Corpus 1,276 assessed · 1,177 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJEB6112

BioProject first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina Genome Analyzer IIx short-read RNA-seq of Mus musculus produces 35.2 billion bases across 204 million reads at 87.5% Q20 and 81.1% Q30. Older-generation platform quality is moderate; data adequate for gene expression surveys though Q-score degradation increases false-positive SNP calls if SNP discovery is intended.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
4 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 35239955980 reported
total reads 204883465 reported
n content pct 0.69 measured
pct q20 bases 87.5 measured
pct q30 bases 81.1 measured
gc content pct 49.7 measured
mean read length 86 measured
mean base quality 32.7 measured
adapter content pct 0.57 measured
duplication rate pct 18.15 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.1 measured ×1 56%
mean base quality 32.7 measured ×0.6 78%
adapter content pct 0.57 measured ×0.4 100%
duplication rate pct 18.15 measured ×0.4 100%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0