PRJEB6358

BioProject first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

49/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina Genome Analyzer IIx 16S/18S amplicon sequencing of human gut microbiota produces 45.8 billion bases at 95% Q20 across 654 million reads. High coverage enables comprehensive taxonomic composition and alpha/beta-diversity analysis; marker-gene approach addresses community structure rather than functional potential.

Data type / assay

amplicon

Organism

human gut metagenome

Instrument

Illumina Genome Analyzer IIx

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1,658 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 45827246739 reported

total reads 654171878 reported

n content pct 0.02 measured

pct q20 bases 95 measured

pct q30 bases 74.8 measured

gc content pct 50.5 measured

mean read length 36 measured

mean base quality 30.2 measured

adapter content pct 0.01 measured

duplication rate pct 78.34 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 49/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.8 measured ×1 24%

adapter content pct 0.01 measured ×0.5 100%

QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0