PRJNA202104

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

55/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Marine amplicon (454 pyrosequencing). Marginal reusability (grade F) with Q30 at only 76.6% (scored 33/100)—at the lower boundary for taxonomic surveys. Platform is now deprecated and base quality is insufficient for abundance-precision work; rough functional summaries only.

Data type / assay

amplicon

Organism

marine metagenome

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 142940625 reported

total reads 273168 reported

n content pct 0.006 measured

pct q20 bases 92.6 measured

pct q30 bases 76.6 measured

gc content pct 45 measured

mean read length 311 measured

mean base quality 34.1 measured

adapter content pct 0 measured

duplication rate pct 96.29 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 55/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.6 measured ×1 33%

adapter content pct 0 measured ×0.5 100%

QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0