PRJNA20341

BioProject first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

39/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina Genome Analyzer II transcriptomics of Capsaspora owczarzaki (a unicellular eukaryote related to animal-fungal divergence) yields 38.1 billion bases with elevated N-content (4.3%) and reduced Q-scores (73.4% Q20). Limited by lower sequence quality, best suited for comparative transcriptome surveys rather than precision isoform quantification.

Data type / assay

bulk-RNA-seq

Organism

Capsaspora owczarzaki ATCC 30864

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

20 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 38109274472 reported

total reads 250718911 reported

n content pct 4.334 measured

pct q20 bases 73.4 measured

pct q30 bases 73.4 measured

gc content pct 52.6 measured

mean read length 76 measured

mean base quality 22.8 measured

adapter content pct 0.03 measured

duplication rate pct 34.79 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 39/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 73.4 measured ×1 17%

mean base quality 22.8 measured ×0.6 0%

adapter content pct 0.03 measured ×0.4 100%

duplication rate pct 34.79 measured ×0.4 89%

QC cost 10 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0