Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA225591

BioProject first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

65/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Spongilla lacustris bulk RNA-seq with poor measured quality (D grade). High adapter contamination at 17.14% and Q30 only 82.1% jointly compromise reliability and introduce false signals. The combination risks spurious low-abundance transcripts and adapter-driven artifacts; recommend extensive trimming and QC filtering, or resequencing.

Data type / assay
bulk-RNA-seq
Organism
Spongilla lacustris
Instrument
Illumina HiSeq 1000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 7008687046 reported
total reads 23207573 reported
n content pct 0.049 measured
pct q20 bases 89.4 measured
pct q30 bases 82.1 measured
gc content pct 47.8 measured
mean read length 151 measured
mean base quality 32.9 measured
adapter content pct 17.14 measured
duplication rate pct 17.99 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 65/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.1 measured ×1 61%
mean base quality 32.9 measured ×0.6 82%
adapter content pct 17.14 measured ×0.4 16%
duplication rate pct 17.99 measured ×0.4 100%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0