Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA227062

BioProject first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

34/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina MiSeq 16S/18S amplicon profiling of human gut microbiota yields 7.6 billion bases at 80.9% Q20 across 15.3 million reads. Adequate for taxonomic composition analysis; lower Q-scores typical of amplicon sequencing but manageable for operational taxonomic unit/amplicon sequence variant calling.

Data type / assay
amplicon
Organism
human gut metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
94 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 7607541396 reported
total reads 15305004 reported
n content pct 0.008 measured
pct q20 bases 80.9 measured
pct q30 bases 71.2 measured
gc content pct 52.5 measured
mean read length 248.7 measured
mean base quality 31.4 measured
adapter content pct 2.42 measured
duplication rate pct 36.54 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 34/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 71.2 measured ×1 6%
adapter content pct 2.42 measured ×0.5 90%
QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0