PRJNA242424

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

63/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read amplicon of human gut metagenome scoring D-grade (63/100), limited by Q30 at 78.9% (measured, scored 44/100) and elevated N-content (0.177%, measured)—unusual for amplicon and suggesting reads of uncertain origin or primer mispriming. Expected high duplication (92.6%) is amplicon-appropriate; low Q30 and N anomaly restrict reuse to low-stringency profiling only.

Data type / assay

amplicon

Organism

human metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

76 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 9822603943 reported

total reads 36388767 reported

n content pct 0.177 measured

pct q20 bases 86.5 measured

pct q30 bases 78.9 measured

gc content pct 53.2 measured

mean read length 250 measured

mean base quality 32.9 measured

adapter content pct 0.06 measured

duplication rate pct 92.6 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 63/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.9 measured ×1 45%

adapter content pct 0.06 measured ×0.5 100%

QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0