Corpus 1,276 assessed · 1,177 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA243055

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

16S rRNA amplicon sequencing of uncultured eukaryotes using 454 GS FLX Titanium, generating ~429k long reads (~500 bp each) with 88.7% Q30. This dataset enables long-read amplicon profiling of eukaryotic microorganisms at higher taxonomic resolution than short-read methods. Long-read sequencing is particularly valuable for this target as rRNA length variants aid species discrimination in eukaryotic communities.

Data type / assay
amplicon
Organism
uncultured eukaryote
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads)
N numbers (samples, groups)
36 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 211230034 reported
total reads 429492 reported
n content pct 0.007 measured
pct q20 bases 96.8 measured
pct q30 bases 88.7 measured
gc content pct 43.1 measured
mean read length 400.3 measured
mean base quality 36.9 measured
adapter content pct 0 measured
duplication rate pct 95.04 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.7 measured ×1 94%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0