Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA250530

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Fungal bulk RNA-seq from Exserohilum turcicum achieves B-grade QC (86/100) with strong base quality (Q30=94%) but one critical issue. Adapter content of 16.59% scored only 19/100 and dominates the grade penalty—incomplete trimming can artificially inflate transcript counts and should be remediated before quantification. Reuse is possible with upstream adapter-trimming after re-QC.

Data type / assay
bulk-RNA-seq
Organism
Exserohilum turcicum
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
27 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 361303068900 reported
total reads 1204343563 reported
n content pct 0.01 measured
pct q20 bases 97.7 measured
pct q30 bases 94 measured
gc content pct 50.5 measured
mean read length 150 measured
mean base quality 35.6 measured
adapter content pct 16.59 measured
duplication rate pct 16.84 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94 measured ×1 100%
mean base quality 35.6 measured ×0.6 100%
adapter content pct 16.59 measured ×0.4 19%
duplication rate pct 16.84 measured ×0.4 100%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0