Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJNA250734

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

59/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of fungus scoring F-grade (59/100) due to dual deficiencies: Q30 at 78.9% (measured) and 10.79% adapter content (measured). Both metrics point to library or sequencing stress; while Q30 borders acceptable for RNA-seq, one-in-ten reads carrying adapters is significant contamination requiring aggressive trimming before expression quantification.

Data type / assay
bulk-RNA-seq
Organism
Penicillium raistrickii ATCC 10490
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 30099746100 reported
total reads 100332487 reported
n content pct 0.004 measured
pct q20 bases 89.1 measured
pct q30 bases 78.9 measured
gc content pct 53 measured
mean read length 150 measured
mean base quality 31.6 measured
adapter content pct 10.79 measured
duplication rate pct 27.03 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 59/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.9 measured ×1 45%
mean base quality 31.6 measured ×0.6 60%
adapter content pct 10.79 measured ×0.4 51%
duplication rate pct 27.03 measured ×0.4 100%
QC cost 45 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0