PRJNA252973

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

49/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Gut amplicon. Unsuitable for reuse; grade F despite measured metrics due to Q30 depth of 74.7% (scored 24/100)—falling well below practical thresholds. Even the favorable duplication at 92.38% cannot overcome base-quality floor that is too low for reliable abundance or variant inference.

Data type / assay

amplicon

Organism

gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

42 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 787682502 reported

total reads 1571363 reported

n content pct 0.005 measured

pct q20 bases 86.8 measured

pct q30 bases 74.7 measured

gc content pct 54.5 measured

mean read length 251 measured

mean base quality 32.1 measured

adapter content pct 0 measured

duplication rate pct 92.38 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 49/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.7 measured ×1 24%

adapter content pct 0 measured ×0.5 100%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0