Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA253870

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

81/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Fungal bulk RNA-seq from Escovopsis weberi achieves B-grade QC (81/100) with multiple concerns. Duplication is high at 51% (53/100 score) and Q30 bases below-ideal at 85.1% (76/100 score, weight 1)—together suggesting over-amplification or suboptimal sequencing, reducing transcript abundance accuracy. Reuse requires validated bias-correction or deduplication; not recommended for quantification-sensitive applications without remediation.

Data type / assay
bulk-RNA-seq
Organism
Escovopsis weberi
Instrument
454 GS FLX Titanium
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
4 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 15977019929 reported
total reads 79762615 reported
n content pct 0.003 measured
pct q20 bases 95.4 measured
pct q30 bases 85.1 measured
gc content pct 55.7 measured
mean read length 100 measured
mean base quality 33.8 measured
adapter content pct 0 measured
duplication rate pct 51 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 81/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.1 measured ×1 76%
mean base quality 33.8 measured ×0.6 97%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 51 measured ×0.4 53%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0