Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA254017

BioProject first seen 2014

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (101bp, HiSeq 2000, Lassa mammarenavirus). Grade B (80/100): usable but library-prep compromised. Adapter content (12.95%) and duplication (56.07%) are the primary drivers—high adapter indicates incomplete trimming, while 56% duplication reduces effective depth by more than half, limiting power for rare transcript detection.

Data type / assay
bulk-RNA-seq
Organism
Mammarenavirus lassaense
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
892 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 492781463073 reported
total reads 2398339052 reported
n content pct 0.001 measured
pct q20 bases 97.3 measured
pct q30 bases 95 measured
gc content pct 50.9 measured
mean read length 101 measured
mean base quality 36.3 measured
adapter content pct 12.95 measured
duplication rate pct 56.07 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95 measured ×1 100%
mean base quality 36.3 measured ×0.6 100%
adapter content pct 12.95 measured ×0.4 39%
duplication rate pct 56.07 measured ×0.4 42%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 78