Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA262180

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Short-read amplicon of zebrafish scoring D-grade (67/100) with 20.12% adapter content (measured) as the primary bottleneck—one-in-five reads affected, reducing effective yield. High duplication (88.58%) is amplicon-expected; the adapter contamination, not duplication, drives reuse constraints. Mean target coverage is extrapolated; a deep measured pass is pending.

Data type / assay
amplicon
Organism
Danio rerio
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
3 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 22184183400 reported
total reads 36973639 reported
n content pct 0.002 measured
pct q20 bases 97.4 measured
pct q30 bases 93 measured
gc content pct 47.2 measured
mean read length 300 measured
mean base quality 36.2 measured
adapter content pct 20.12 measured
duplication rate pct 88.58 measured
mean target coverage 739.5 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%
adapter content pct 20.12 measured ×0.5 0%
QC cost 40 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0