PRJNA270876

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

miRNA-seq of human tissue scoring D-grade (67/100) with catastrophic library failure: 96.77% adapter content (measured, scored 0/100) and 86.54% duplication (measured, scored 0/100). The read set is predominantly sequencing adapters, not miRNA inserts; not reusable without library reconstruction. Indicates failed library construction or QC filtering malfunction.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina Genome Analyzer IIx

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

15 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3531938212 reported

total reads 60170723 reported

n content pct 0.015 measured

pct q20 bases 97.7 measured

pct q30 bases 94.1 measured

gc content pct 48.4 measured

mean read length 66 measured

mean base quality 37.3 measured

adapter content pct 96.77 measured

duplication rate pct 86.54 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.1 measured ×1 100%

mean base quality 37.3 measured ×0.6 100%

adapter content pct 96.77 measured ×0.4 0%

duplication rate pct 86.54 measured ×0.4 0%

QC cost 44 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0