Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA272543

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

43/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Hypsibius dujardini (tardigrade/water bear) via HiSeq 2000 with ~302M short reads, but notably very high N content (0.453%) and lower base quality (74.1% Q30). This dataset captures the transcriptome of an extremophile organism adapted to desiccation and radiation resistance. The high N content and moderate quality suggest data quality issues, requiring aggressive quality filtering before reliable transcript quantification and assembly.

Data type / assay
bulk-RNA-seq
Organism
Hypsibius dujardini
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
126 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 10583229930 reported
total reads 302377998 reported
n content pct 0.453 measured
pct q20 bases 81.3 measured
pct q30 bases 74.1 measured
gc content pct 42.9 measured
mean read length 35 measured
mean base quality 30.4 measured
adapter content pct 0 measured
duplication rate pct 53.6 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 43/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.1 measured ×1 21%
mean base quality 30.4 measured ×0.6 40%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 53.6 measured ×0.4 48%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0