PRJNA273396

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Zebrafish amplicon with excellent Q30 purity (93.1%, measured) and zero adapter contamination, excellent for metabarcoding. Target coverage is extrapolated; measured validation pending, but Q30 and adapter metrics are strong.

Data type / assay

amplicon

Organism

Danio rerio

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4883573498 reported

total reads 12271911 reported

n content pct 0.261 measured

pct q20 bases 95.2 measured

pct q30 bases 93.1 measured

gc content pct 49.3 measured

mean read length 224.7 measured

mean base quality 36.2 measured

adapter content pct 0 measured

duplication rate pct 97.26 measured

mean target coverage 162.8 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.1 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 46 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0