Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA273417

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

61/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

16S rRNA amplicon sequencing of bovine (cattle) gut metagenome via 454 GS FLX Titanium long-read platform with ~216k reads spanning ~115 Mb. This dataset enables long-read amplicon profiling of ruminant microbiota at high taxonomic resolution, particularly valuable for detecting rRNA length variants that define species boundaries in anaerobic bacteria. Long-read sequencing captures the full 16S gene length for improved phylogenetic accuracy.

Data type / assay
amplicon
Organism
bovine gut metagenome
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads)
N numbers (samples, groups)
48 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 114500975 reported
total reads 216352 reported
n content pct 0.012 measured
pct q20 bases 93.4 measured
pct q30 bases 78.2 measured
gc content pct 53.2 measured
mean read length 483.7 measured
mean base quality 34.6 measured
adapter content pct 0 measured
duplication rate pct 82.51 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 61/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.2 measured ×1 41%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0