PRJNA273894

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon (Illumina MiSeq, Mus musculus) with perfect base quality (100/100, grade A) but limited practical depth. Perfect Q30 (100%) and zero adapters are exceptional, yet the 98.34% duplication and extrapolated coverage (62.6×) indicate a tightly focused panel with minimal template diversity. Best for presence/absence detection rather than quantitative work.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3379105995 reported

total reads 26250878 reported

n content pct 0.542 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 52.4 measured

mean read length 128.6 measured

mean base quality 30 measured

adapter content pct 0.14 measured

duplication rate pct 98.34 measured

mean target coverage 62.6 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

adapter content pct 0.14 measured ×0.5 100%

QC cost 10 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0