Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA274725

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

RNA-seq severely compromised by duplicate reads (70.76%, measured) inflating transcript abundance, with secondary adapter contamination (4.79%, measured). Requires aggressive deduplication and trimming; post-processing overhead makes dataset marginal.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
6 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 34834388940 reported
total reads 172447470 reported
n content pct 0.001 measured
pct q20 bases 96 measured
pct q30 bases 91.4 measured
gc content pct 56 measured
mean read length 101 measured
mean base quality 35.1 measured
adapter content pct 4.79 measured
duplication rate pct 70.76 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.4 measured ×1 100%
mean base quality 35.1 measured ×0.6 100%
adapter content pct 4.79 measured ×0.4 85%
duplication rate pct 70.76 measured ×0.4 9%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0