Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA274794

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

E. coli reference WGS of B grade; while pct_q30_bases=100% (measured) and adapter content is low (3.69%, measured), the elevated duplication rate (23.64%, measured) suggests possible PCR bias or contamination and will compress coverage uniformity. All metrics measured with evidence_strength=1; suitable for reference genome validation and comparative genomics with caveats on coverage distribution.

Data type / assay
WGS
Organism
Escherichia coli str. K-12 substr. MG1655
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
20 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 6182556300 reported
total reads 82434084 reported
n content pct 0.011 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 49.2 measured
mean read length 75 measured
mean base quality 30 measured
adapter content pct 3.69 measured
duplication rate pct 23.64 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
duplication rate pct 23.64 measured ×0.5 51%
adapter content pct 3.69 measured ×0.4 81%
QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0