PRJNA276803

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Hot springs metagenome, 454 long-read amplicon; grade F—unsuitable for reliable profiling. Q30 of 69.4% (the lowest among all datasets) means >30% of bases are Q<30 errors, substantially inflating community composition estimates. High duplication (49.16%) adds further uncertainty to ecological abundance calls.

Data type / assay

amplicon

Organism

hot springs metagenome

Instrument

454 GS FLX+

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

12 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 23251472 reported

total reads 42160 reported

n content pct 0.006 measured

pct q20 bases 90.1 measured

pct q30 bases 69.4 measured

gc content pct 42.7 measured

mean read length 469.7 measured

mean base quality 33.2 measured

adapter content pct 0 measured

duplication rate pct 49.16 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 69.4 measured ×1 0%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0