Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJNA279814

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Very large-scale bulk RNA-seq of Eufriesea mexicana (a large carpenter bee) via HiSeq 2000 with ~681M short reads spanning ~130 Gb at 81.3% Q30. This dataset enables comprehensive transcriptome characterization of a solitary bee species with detailed capture of tissue-specific and behavioral gene expression. The exceptional read depth supports isoform discovery and detection of lowly-expressed regulatory transcripts despite moderate Q30 rate.

Data type / assay
bulk-RNA-seq
Organism
Eufriesea mexicana
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
10 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 130318713340 reported
total reads 681146111 reported
n content pct 0 measured
pct q20 bases 88 measured
pct q30 bases 81.3 measured
gc content pct 42.8 measured
mean read length 100 measured
mean base quality 32.5 measured
adapter content pct 0.11 measured
duplication rate pct 2.07 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.3 measured ×1 56%
mean base quality 32.5 measured ×0.6 75%
adapter content pct 0.11 measured ×0.4 100%
duplication rate pct 2.07 measured ×0.4 100%
QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0