Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA279815

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Whole-genome amplification (WGA) sequencing of Escherichia coli BL21 (a common laboratory strain) via MiSeq with ~8.5M short reads at moderate quality (81.5% Q30). This dataset captures amplified genomic DNA from a widely-used protein expression host, useful for strain comparison and laboratory stock verification. The WGA-induced bias and lower Q30 rate warrant careful quality filtering before variant calling or assembly.

Data type / assay
WGS
Organism
Escherichia coli BL21
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
24 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4276210696 reported
total reads 8518348 reported
n content pct 0.001 measured
pct q20 bases 86.3 measured
pct q30 bases 81.5 measured
gc content pct 36.2 measured
mean read length 251 measured
mean base quality 33.6 measured
adapter content pct 65.7 measured
duplication rate pct 36.25 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.5 measured ×1 57%
duplication rate pct 36.25 measured ×0.5 12%
adapter content pct 65.7 measured ×0.4 0%
QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0