Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJNA279825

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Dufourea novaeangliae (sweat bee) via HiSeq 2000 with ~229M short reads at 84% Q30, spanning ~43 Gb of sequence data. This dataset enables comprehensive transcriptome profiling of a solitary bee species relevant to understanding social behavior evolution and immune function. The substantial read depth despite moderate Q30 supports isoform reconstruction and detection of behavioral-associated genes.

Data type / assay
bulk-RNA-seq
Organism
Dufourea novaeangliae
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
5 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 43491049240 reported
total reads 229408487 reported
n content pct 0.003 measured
pct q20 bases 91.1 measured
pct q30 bases 84 measured
gc content pct 48.5 measured
mean read length 90 measured
mean base quality 33.3 measured
adapter content pct 0.45 measured
duplication rate pct 48.42 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84 measured ×1 70%
mean base quality 33.3 measured ×0.6 88%
adapter content pct 0.45 measured ×0.4 100%
duplication rate pct 48.42 measured ×0.4 59%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0