PRJNA290483

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Gut 16S amplicon with good base quality and low contamination. Strong QC (96/100, A) with Q30 of 88.9% and minimal adapter content (0.04%), supporting accurate taxonomy; the high duplication (94.81%) is appropriate for the highly-conserved 16S target and does not compromise the clean reads. Suitable for microbial community profiling and relative abundance studies.

Data type / assay

amplicon

Organism

gut metagenome

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

55 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 34856501850 reported

total reads 689853744 reported

n content pct 0 measured

pct q20 bases 92.6 measured

pct q30 bases 88.9 measured

gc content pct 55 measured

mean read length 250 measured

mean base quality 34.5 measured

adapter content pct 0.04 measured

duplication rate pct 94.81 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.9 measured ×1 95%

adapter content pct 0.04 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0