PRJNA294127

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing of sheep achieving perfect score despite 85.5% duplication inherent to amplicon design: Q30 96.2%, mean base quality 37, zero N content, and minimal adapter (0.03%) exemplify excellent amplicon protocol execution. Excellent for reuse in amplicon analyses.

Data type / assay

amplicon

Organism

Ovis aries

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

35 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 339500018 reported

total reads 687247 reported

n content pct 0 measured

pct q20 bases 98.3 measured

pct q30 bases 96.2 measured

gc content pct 52.5 measured

mean read length 246 measured

mean base quality 37 measured

adapter content pct 0.03 measured

duplication rate pct 85.5 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.2 measured ×1 100%

adapter content pct 0.03 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0