Corpus 1,276 assessed · 1,177 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA296743

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Alicyclobacillus acidoterrestris (thermophilic bacterium) via MiSeq with modest ~12M short reads at 88.4% Q30. This dataset captures bacterial transcriptomics of an extreme thermophile relevant to understanding adaptation to hot environments. The smaller read count suggests focused sampling of specific conditions, requiring verification of experimental design for reuse decisions.

Data type / assay
bulk-RNA-seq
Organism
Alicyclobacillus acidoterrestris
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
6 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1854063250 reported
total reads 11980801 reported
n content pct 0.002 measured
pct q20 bases 94.5 measured
pct q30 bases 88.4 measured
gc content pct 46.2 measured
mean read length 80 measured
mean base quality 34.7 measured
adapter content pct 3.4 measured
duplication rate pct 75.19 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.4 measured ×1 92%
mean base quality 34.7 measured ×0.6 100%
adapter content pct 3.4 measured ×0.4 92%
duplication rate pct 75.19 measured ×0.4 0%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0