PRJNA297869

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

35/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

WGS (human gut metagenome) with catastrophic quality failure (35/100, grade F). Q30 at 66.8% (scoring zero) and mean base quality of 27.7 are far below ~85%+ benchmarks; 5.19% residual adapters compound the damage. Do not reuse; obtain fresh sequencing rather than attempting salvage.

Data type / assay

WGS

Organism

human gut metagenome

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

27 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2187294834 reported

total reads 21444067 reported

n content pct 0.002 measured

pct q20 bases 74.2 measured

pct q30 bases 66.8 measured

gc content pct 44.7 measured

mean read length 51 measured

mean base quality 27.7 measured

adapter content pct 5.19 measured

duplication rate pct 15.64 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 35/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 66.8 measured ×1 0%

duplication rate pct 15.64 measured ×0.5 76%

adapter content pct 5.19 measured ×0.4 70%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0